What are the best shopping carts you know in terms of visual design and usability.
In addition, provide best practice tips when designing a shopping cart as @scunliffe did.
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I'm not sure I know of the best UI's but I know the ones that frustrate the heck out of me ;-) Logic would suggest that the opposite features are the ones that make it better.
I'd do some research over at Design Meltdown. He's collected lots of eCommerce sites, and I'll bet the do alot of different cart designs. Experience them, see which ones works best.
Be aware of business goals vs. usability goals. For instance some stores create a "funnel", where once you are on the checkout pages there is no more nav for the rest of the site. The idea is to not distract you from the buying process so you finish. This is a business goal, and as a user I find it frustrating. What if I forgot something and want to go try to find it to add it to my cart? Sometimes I have to actually hack the URL to get back to the main site again.
If using a multi-step wizard, make it easy to go back and change information that you entered earlier that you now realize is wrong once you get to the confirm page. Do not make the user click Back, Back, Back and then Next, Next, Next. If possible, create a one-page checkout, or let the user edit any information on the last page on the fly.
Do not require a lot of info from the user to create an account just to save their shopping cart or wishlist.